A self assembled dextran-stearic acid-spermine nanocarrier for delivery of rapamycin as a hydrophobic drug

Over the past decades, natural polysaccharides have received considerable attention to developing nano-drug delivery systems for of anticancer drugs. However, low solubility these agents within biological fluid has reduced their efficacy in inhibiting growth cancer cells. In this study, self-assembled cationic based on dextran-stearic acid-spermine conjugates (D-SASp) were synthesized, and potential as carriers sustained release rapamycin was demonstrated. Nanomicelles (smaller than 200 nm) with relatively narrow size distribution obtained through dialysis D-SASp solution DMSO against distilled water. The from nanocarriers occurred without initial burst release. vitro toxicity studies U87 MG cell line confirmed that synthesized polymers not toxic up 0.4 mg/mL, cytotoxicity rapamycin-loaded micelles higher free rapamycin. Furthermore, flow cytometry analysis fully supported time-dependent cellular uptake nanocarriers. addition, presence conjugated spermine a positive charge resulted increased transport nanomicelles into